FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677139
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG30520.4507198669697064No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA20670.3052549033507153No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA19760.2918160082346461No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATACCT11320.16717394803725674No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA10620.1568363364095112No Hit
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTG10460.1544734537517408No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC9970.1472371256123189No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA9430.13926239664234374No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATCT8490.12538046102794256No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG8130.12006397504795913No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)