FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359028
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG48150.3542973360372266No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA34290.2523126822994081No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA29470.21684615769505855No Hit
GTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAGAACACAA20120.14804698652272066No Hit
TATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGC19650.14458863246379028No Hit
ATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCA18110.13325700427069934No Hit
ATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGATACCT15840.1165538899860783No Hit
ACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAGCACCAG15270.11235971591460957No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC15110.11118240389454816TruSeq Adapter, Index 8 (95% over 23bp)
GTTCTGGTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTG13740.10110166972277246No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)