FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780685
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25560.32740477913627136No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG22550.28884889552124093No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT20370.26092470074357776No Hit
ATATGATACTTCACCGGGGACGTTCGGCCACGGGACCAAGGTGGATATGAAACGAACTGTGGCTGCACCATCTGT20180.2584909406482768No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC18680.239277045159059No Hit
GCATAAATCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGTAGGGCCATTGGCATCCCAGCCAGGTT18440.2362028218807842No Hit
CCTTGGTCCCGTGGCCGAACGTCCCCGGTGAAGTATCATATTGTTGGCAGTAATAAATCCCAAAATCCTCAGGCT17680.2264677814995805No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAG17450.22352165085790043No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA17410.22300928031152129No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA16440.21058429456182715No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTC15760.2018739952733817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG15440.1977750309023486No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG14980.19188276961898845No Hit
GGACCAAGGTGGATATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA14930.1912423064360145No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATA14870.1904737506164458No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGTGATAGGGCCACCCTCTCATGTTGGGCCAGTCAGACCGTTAGTAG14480.18547813778924918No Hit
CAATATGATACTTCACCGGGGACGTTCGGCCACGGGACCAAGGTGGATATGAAACGAACTGTGGCTGCACCATCT14130.1809948955084317No Hit
GATTTATGCTGGTACCAGGCTAAGTAACTACTACTAACGGTCTGACTGGCCCAACATGAGAGGGTGGCCCTATCA14050.1799701544156734No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATATCCACCTTGGTCCCGTGGCCGAACGTCCCCGGTGAAGT13840.17728020904718292No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13270.16997892876128015No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13000.16652042757322097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12590.16126862947283474No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12100.15499209027969027No Hit
TCTCTGGTGCATCCAGTAGGGCCATTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAAATTTCACTC11950.1530707007307685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11820.1514054964550363No Hit
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA11700.14986838481589887No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG11660.14935601426951972No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTC11600.148587458449951No Hit
GTATCATATTGTTGGCAGTAATAAATCCCAAAATCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAATTTGTC11570.14820318054016665No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC11440.14653797626443443TruSeq Adapter, Index 1 (95% over 23bp)
CTCCAGGCACCCTGTCTTTGTCTCCAGGTGATAGGGCCACCCTCTCATGTTGGGCCAGTCAGACCGTTAGTAGTA11430.14640988362783966No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAG11260.1442323088057283No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11050.1415423634372378No Hit
CACCATCACCAGACTGGAGCCTGAGGATTTTGGGATTTATTACTGCCAACAATATGATACTTCACCGGGGACGTT10730.13744339906620467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10700.13705912115642033No Hit
GCCTGGTACCAGCATAAATCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGTAGGGCCATTGGCATC10480.13424108315133504No Hit
ATCATATTGTTGGCAGTAATAAATCCCAAAATCCTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAATTTGTCCC10320.13219160096581847No Hit
CAGTAGGGCCATTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAAATTTCACTCTCACCATCACCAG10320.13219160096581847No Hit
GTGCAGCCACAGTTCGTTTCATATCCACCTTGGTCCCGTGGCCGAACGTCCCCGGTGAAGTATCATATTGTTGGC10260.13142304514624975No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT10250.13129495250965498No Hit
GTCTTTGTCTCCAGGTGATAGGGCCACCCTCTCATGTTGGGCCAGTCAGACCGTTAGTAGTAGTTACTTAGCCTG10140.12988593350711236No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGA9910.12693980286543227No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT9520.12194419003823564No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAAACGGAGAAATTATATTGACGCAGTCTC9460.12117563421866695No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC9310.11925424466974516No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG9130.11694857721103902No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG9090.11643620666465988No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTC9090.11643620666465988No Hit
CACCAGAGATGAGGAGCCTGGGAGCCTGGCCAGATTTATGCTGGTACCAGGCTAAGTAACTACTACTAACGGTCT9090.11643620666465988No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA9060.11605192875487552No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9050.11592383611828073No Hit
CTCTCATGTTGGGCCAGTCAGACCGTTAGTAGTAGTTACTTAGCCTGGTACCAGCATAAATCTGGCCAGGCTCCC8910.11413053920595374No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8890.11387435393276418No Hit
CCTCATCTCTGGTGCATCCAGTAGGGCCATTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAAATTT8850.11336198338638503No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8750.11208105702043719No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8610.1102877601081102No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA8560.10964729692513625No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGAGGACGGGAGGCCTTTG8470.1084944631957832No Hit
GTCTGGTGATGGTGAGAGTGAAATTTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAATGGCCCTAC8460.10836637055918841No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC8440.10811018528599882No Hit
ATACTTCACCGGGGACGTTCGGCCACGGGACCAAGGTGGATATGAAACGAACTGTGGCTGCACCATCTGTCTTCA8440.10811018528599882No Hit
TCACCAGACTGGAGCCTGAGGATTTTGGGATTTATTACTGCCAACAATATGATACTTCACCGGGGACGTTCGGCC8360.10708544419324056No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8170.10465168409793962No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8060.103242665095397No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC7910.1013212755464752No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)