FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560696
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC75810.29605232327460973RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG44010.1718673360680065No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA30660.1197330725708948No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)