FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282842
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC12410.43876086295528954Illumina PCR Primer Index 10 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG6440.22768895708558134No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT5990.21177901443208574No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT4600.16263496934684382No Hit
TAGTAACCCATTAGAATGGATAAAAACACTTGGAAGCTCTGTATTTCAATGATGATTGTGCTTTTAATCCATGTT4300.1520283409111801No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG3730.131875746883419No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC3550.12551176982202078No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3120.11030893573090277No Hit
GGATGCAGGGGGGGTGATCATGTACGAGCCATGGCACTCCTCATTGGCAATCCCAAGAGAGACTTTTAGCCAGGC3060.10818761004377No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG2860.1011165244199942No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)