FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004048055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6183745
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG154440.2497515664051477No Hit
GGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCAGACGCAGCGGGCGTGCTTGTGGACTGCTTG130290.21069756272291307No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT120810.19536704699175014No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC107640.17406927355510293TruSeq Adapter, Index 6 (95% over 22bp)
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG86960.140626756116237No Hit
CTCATCAGATAAGCTGTCATTGGAAGGAATAGTGGTACAAAGAGCTGAATGCCGACCAGCTGCCAGTGAAAACTA75210.121625325753245No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC73600.11902172550776269No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTG71170.11509206799439498TruSeq Adapter, Index 6 (95% over 21bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA66340.10728126725794807No Hit
GACTACAGGCACATGCCACCACACCCAACTAATTTATGTACTTTTTACTAGAGATGGGGTTGTGCCATGTTGGCC64980.10508195276486983No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)