FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499990
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC300911.2036448145792582Illumina PCR Primer Index 7 (95% over 21bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTG95640.382561530246121No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCG61640.24656098624394499No Hit
ACACAAGTCCCAGTGTGGCATCACCAGAACACTCAGTGGAATCTTCCAGCTATGGAGACACACTACTTGGCCTCA48990.19596078384313537No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG45300.1812007248028992No Hit
TTCCACAGCTTTGAAGGCCCCTTTGAGGTGGCTGGAGGGGCCCAGAGTGGTCGGGGAGAGACTTTGCTGGGCAGC41780.16712066848267393No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTA39460.15784063136252546No Hit
GATGTTGTACACCTTACGGTCGATCACTAGCCACCGCTCCTCGCACCCTGAGCGCTGGGCCACCTCGTCCCAGGT38440.15376061504246016No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT36950.1478005912023648No Hit
TTGCATGACTAATCGTTGACATTCTTAAGATGAACTGGATGCTGCAACAGCTGCCCTCTTGGGTTTAGGTGTTGT36100.1444005776023104No Hit
CCTAGGAGCCAGGTCCTACGGATAGGTGAGCCGGCCCCCCAGGGCAAACACCTCCACTGGGCAGACCTGACTGCC33770.1350805403221613No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG33010.13204052816211265No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC32300.1292005168020672No Hit
GGAAGGGTCCAGGCAAAAGAAGAAGATGGCTCGGACCCTTGGGTGTGATGTGGGTGGCACCAGGGGCTGGGATCC31310.12524050096200384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)