FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153670
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC170041.4739050161658014RNA PCR Primer, Index 47 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG33680.2919379025197847No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT28900.25050491041632356No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTG27840.24131684103773174RNA PCR Primer, Index 47 (95% over 22bp)
CCCAAGACTTCCAGAAGGGAAGGGGAGGAGGGGCAAAGCACTGTGCCTACCAGGTGCTGCCTGCCCCGTCCTGCT25600.22190054348297175No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATG22590.19580989364376294No Hit
ATGGAAGCCCGCCAAACCTTCAACTCCCTGGCAGCCCTGGTGAATGGTATCCCATTCAACAGCTATAGGGTGGCT18370.15923097592899182No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17060.14787590905544912No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC16940.1468357502578727No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCG16660.1444087130635277No Hit
GTACATGGGACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTT16520.1431951944663552No Hit
AACCAAGTCTTTAATACTTAGATGTTGATTTAGTATAAATCATTCCTATGGTCTGATACAGCTTTATGAATACTT16150.13998803817382785No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG16060.13920791907564553No Hit
CTTCTGCCATCTCCTTGGCAAACTCCAAGTCCTGCTGTTCTCGCTCACGCCGTTCCTCTGCAGACTCCAAGTCCT15750.13652084218190644No Hit
TCCATGAGGGATTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC14300.12395225671119124No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA14120.12239201851482659No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG13940.12083178031846195No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG12430.10774311544895854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)