FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107968
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC242761.151630385280991TruSeq Adapter, Index 7 (95% over 22bp)
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTG71300.3382404286972098TruSeq Adapter, Index 7 (95% over 21bp)
CAAGAAAACACATGAAACCCTGAGTCACGCAGGGCAAAAGGCAACTGCAGCTTTCAGCAACGTTGGAACGGCCAT53840.25541184685915536No Hit
GCAATCGCAGGCACTGACGACTCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCG36510.17319997267510703No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG36460.1729627774235662No Hit
CGGAGACGCGTCACCAGCCCGCTCCCAGCATCCCGCTCCGTGAAGGCCGACCGCTCCGTGACTGGGTCCTCAGGT32580.15455642590399854No Hit
GTCTTCCAGAGAGTTTACCTCTCATTCAGGGGCCAGTGGGTTTGGATCAAAGGTTTTCACTAAAACAGATTGAAG30430.14435703008774328No Hit
CGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC29820.14146324801894525No Hit
TCCTTATCCTTTCTGTTCCTCTCAAGATGCTTTCGAACAGCAACTGCTTTCTTAATTAAATGGTAGAGATCTTCA27190.1289867777878981No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG24090.11428067219236725No Hit
GGCCAAGATCTCCACGCAGGCCGACACCATCGGCACCGAGACTCTGGAGAGGCTCTTCCTCAGCCACCCGCAGAC23350.1107701824695631No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA22830.10830335185353858No Hit
AAAGAAAAGAGGTTCATTTGGCTCAAGGTTCTACAGGCTGTACAGGAAACATGACATCAGCATCAGCCTCTGATG22040.10455566687919361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)