FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004048064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876954
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC173540.4476194455750571RNA PCR Primer, Index 41 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG129940.3351600251124981No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT104750.270186337005804No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTG86560.22326806044126393RNA PCR Primer, Index 41 (95% over 22bp)
CCACAAGGATGAGCACGCGGAGTTCAGGCCAGTGTGACTCGGGGGCCACTTGCCGAGCGATGCTGTCCTTCCCGT65640.16930817337528378No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC63250.16314354000589124No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA61590.15886182812589472No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG50260.1296378548726655No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA50020.12901881219122022No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG48990.12636208735001755No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA43290.11165982366569219No Hit
TTTAGAGACAGGGTCGCTATGTCACACAGGCTGGAGTGTGGTGGCACTATCACAGCTCATTGTAGCCTTGGGCTC43230.11150506299533088No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC42560.10977690217629614No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC39240.1012134784163031No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)