FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004048066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4067184
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG92880.22836439167738662No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT71250.17518263250445518No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA66270.16293828850624906No Hit
GGACATGTTAGCTGAAAAACTTCCAAATCTCACACATCTAAACTTAAGTGGAAATAAACTGAAAGATATCAGCAC62910.15467704436288107No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC49160.12086986966903883No Hit
GACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGG46630.11464934952537187No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC45450.11174807926073665TruSeq Adapter, Index 6 (95% over 22bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA42700.10498664432196823No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)