FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004048068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3416622
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC223730.6548280728743185Illumina PCR Primer Index 7 (95% over 21bp)
GCTCTGTGGTCATCAATGATTTCAAATTCGCCAATGTAACCATGCTTCATCATCACAGTGAGAAACCGGACGATG97060.28408176262987245No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG87410.25583749094866215No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTG75700.22156387215208473No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT68940.20177824763757887No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG67370.19718306561276022No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG58890.17236322894367595No Hit
GGCAAGCACACACTAGGGCAGCTGGGCCATCCTGGCCCTAATCCCTCCAGCGGTGTCCACACTGAGCATTGCAGC54010.15808011538882555No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC45870.13425541368053007No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA39170.11464540121792811No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA37930.1110160854785809No Hit
CAGCAGCACAGGCTGAAGTTAGCTGACGCATGCTTTTGGCTTTTATCCCACGGGCGGGTAGTGGCCGGACCCCTG35550.1040501407530596No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)