FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004048099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139615
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG80180.2553816311872634No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG65290.20795543402614652No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT64700.20607622272157572No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC56590.1802450300434926TruSeq Adapter, Index 6 (95% over 22bp)
GAGTTGGACTGTGAAGAAACATGGCGGCCGCGACGTTGACTTCGAAATTGTACTCCCTGCTGTTCCGCAGGACCT46920.14944507527196807No Hit
GGTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATA41970.13367881093700978No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC41300.13154479131995483No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTG38760.12345462739858232TruSeq Adapter, Index 6 (95% over 21bp)
GTGCTATCCAGGCCCAGCAGCGGGTTCGGAATCGGTTTGCCCATGGTGGATCCGCGCCCATGTACTCTGCGTTGA37300.11880437569574613No Hit
CCTCCACTTCCGCCTTCTCCAGGGACTCCAGGACCAGCATGGACAAGTCTGCTGCACTGTTTTGCTGGCCATGGC37060.11803995075829361No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA36190.11526891036002822No Hit
GCCACAGAGTCCCACTTCTGCACAGGATGGGCCTTCAGAGTGATGAGAGAGCATCTCCCGCACAGTGCAAACCCC33830.10775206514174508No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA33190.1057135986418717No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG32270.1027833030483037No Hit
GGCCATGTCCCGCCCTCGCATGCGCCTGGTGGTCACCGCGGACGACTTTGGTTACTGCCCGCGACGCGATGAGGG31520.10039447511876456No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)