FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290426
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG53250.23248950195291185No Hit
ACACGGAGAAGGTGATCGCGGCGTTGCCGGGACTCGAGACCCCGAACCTGACAGGGGGTACCGCCAAGGTACTTC51550.22506730189056534No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT43710.19083786160303803No Hit
GTATCAACGCAGAGTACATGGGCGCGGATCCACCATGGGCAAACCGATTCCGAACCCGCTGCTGGGCCTGGATAG34560.1508889612674673No Hit
GTGTGGACTTGGGAGAGGACTGGGCCATTCTCCTTAGAGAGAAGTGGGGTGGCTTTTAGGATGGCAAGGGACTTC31100.13578260114057383No Hit
GAAGATCAAGCGCCTGAGAAAGAAGTTTGCCCAAAAGATGCTTCGAAAGGCAAGGAGGAAGCTTATCTATGAAAA30930.1350403811343392No Hit
GTGTTGCCTGAGATGATGCATGTGGCAGACGAGGAACCAATGAGTCGGTATCCTTTAGGACAAGAATATTTAATT30860.134734761131772No Hit
GCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC28830.12587178105732294TruSeq Adapter, Index 6 (95% over 22bp)
CTTTTCTTTTCTCTTCTCTCCTTTCTTCAAGTTTTTTTGTTAGTCTTTCCACTCGGATGTTGAGGTCCTCTGCAG28810.12578446105658947No Hit
CGTCAGATGTGTATAAGAGACAGGCGAAGGCTTCCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA28250.12333950103605182No Hit
CTATTAAATACAAAATGGCAGTTTTCTTTCAAAGTGAAAATATTTGCTTCTTTGATACTCCTCAGTGCCTTGCAG27630.12063258101331369No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC26210.11443286096123603No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24290.1060501408908212No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)