FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555178
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC818355.262098615078145No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT259741.6701625151590362TruSeq Adapter, Index 7 (96% over 27bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT84660.5443749847284362No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG50890.32722942325573023No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG50700.3260076981541663TruSeq Adapter, Index 7 (95% over 24bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT35620.2290413058826707No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA32280.20756466462359935TruSeq Adapter, Index 7 (96% over 28bp)
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG31190.20055582061989044No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG27580.17734304369017567No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC26240.16872666665809316No Hit
TTCTAGTACAGATGATTTCATCATCCAGGTATGAAGTCTAGTTCCCATTAGTTGTTTTTCCTGATCATCTCCCTC23510.15117240598825343No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA23330.15001498220782444No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG21300.13696181401743082No Hit
AATCGCTTCTGACATTGCGTTTCTTGGACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCAT21300.13696181401743082No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA19050.12249401676206839No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT18920.12165809958731412TruSeq Adapter, Index 7 (95% over 21bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT18410.11837873220943197TruSeq Adapter, Index 7 (96% over 27bp)
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGC16580.10661159044173722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)