FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632736
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC17093910.469481900319463No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT330792.02598582991984TruSeq Adapter, Index 11 (96% over 29bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT197511.2096872978852673No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG104370.6392337769241322TruSeq Adapter, Index 11 (96% over 26bp)
AAACTAACTGTCAACTGCCTGTGATGGTGGGGAAGCCAGACATCAATCAATCAGGTTTTATCAGACGCTCTCTTC85560.5240283793583286No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG74950.45904543049213103No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG61530.37685210591301965No Hit
GGCCAATATGGTAAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGTGCATGCCTGTAGTCC48620.2977823726554691No Hit
AATCGCTTCTGACATTGCGTTTCTTGGACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT47140.2887178331340768No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT45200.27683593673441387TruSeq Adapter, Index 11 (95% over 23bp)
TGAGAGAGACAGAAGGAGGACGATGACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCT39080.2393528408756835No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA39030.23904660643239323TruSeq Adapter, Index 11 (96% over 30bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG38190.23390186778511649No Hit
TGAGAGAGACAGAAGGGGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT36500.22355114360190503No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT29890.18306695019892988No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG27200.1665915371499128TruSeq Adapter, Index 11 (96% over 26bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT27170.16640779648393864TruSeq Adapter, Index 11 (96% over 29bp)
AATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCTTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGAT24960.1528722340905082No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC21250.13014963839836938No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG21120.12935342884581463TruSeq Adapter, Index 11 (96% over 30bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18360.11244928757619113No Hit
GTCCAATATGGTAAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGTGCATGCCTGTAGTCC17730.1085907335907336No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA17420.10669208004233385TruSeq Adapter, Index 11 (96% over 30bp)
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG16590.10160858828371518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)