FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694367
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC31120.44817798080841975No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG29070.41865468837084713No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT20490.2950889083150553No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC13570.19542979433066374No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12370.17814786705013344No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG11190.16115397189094527No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCC11020.1587056988595368No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA9930.14300794824638843No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG9180.13220674369605698No Hit
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGGC8560.12327774793444964No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA8240.11866923399297488No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCT8230.1185252179323038No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC7660.1103163024740519No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAGGAAGTCAG7630.10988425429203864No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA7600.10945220611002539No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGC7370.1061398367145904No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGA7330.10556377247190607No Hit
CTGGAGGACAGGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTG7240.10426762792586629No Hit
GGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTG7020.10109927459110239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)