FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725216
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC413725.7047831266822575No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT107961.4886599302828398TruSeq Adapter, Index 7 (96% over 27bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT37540.5176388827604466No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG26830.36995874332612627TruSeq Adapter, Index 7 (95% over 24bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACCATCGCAATGCTTTGTTTTA17050.23510236950094868No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG13890.19152914442042096No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG12950.17856748885849183No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA11870.16367537395755197TruSeq Adapter, Index 7 (96% over 28bp)
CTCCTGCCTTCTTCACCTCTTGCTTGGTGGGAGGGCTGTCGGAGGCTGGCACTGCTGTTGGAGATGGCTGGCTGC11850.16339959405197899No Hit
AATCGCTTCTGACATTGCGTTTCTTGGACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCAT10510.14492234037859064No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTT9500.13099545514715616TruSeq Adapter, Index 7 (96% over 27bp)
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACCATCGCAATGCTTTGTTTT8910.12285994793275383No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA7910.10907095265410582No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG7910.10907095265410582No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT7780.10727838326788158TruSeq Adapter, Index 7 (95% over 21bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG7680.10589948374001676TruSeq Adapter, Index 7 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)