FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437951
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT505862.0749391599749134No Hit
CCCATACACTTTCACACACTCAAACTCACACACTAATGTACACCCACACACTCACATATACTTTCACACACAAAC305131.251583809518731No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC272211.1165523835384714No Hit
TCACCCAGGACGGACACACTCACATCGCTGAGAGACAGAGAGGAACCCTGCAGAGCAAGCAACAAATGGTCAACT169180.6939433975498277No Hit
CCCAGTAAGTGACTTGGGAACAAAGGAACACATGTAAGAAGATAAGGCAGCATCAGTTCAGGAGCTTGTCTTGGA165970.6807766029752034No Hit
ACCAAGAAATCACACAGAAAATGTTGAATTTAATCTGTTGCTAGACAAAAGTGAGCTACGGATCATATGGTCTTG157890.6476340172546535No Hit
GAACAAGAGTGACCTTTAGGAGCTGAGAGCAGTTCTCAATTGATAGTGAACAAGAGAATGAGAACCTCAACCCTA136790.5610859283061883No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG127320.5222418334084648No Hit
GTCTGGACTCATTACATTTGCAGAGACCTGATTTCCAAAGAAGGTCACGTTAACAGGTACCTGGCGTCAGAACTT125240.5137100786685213No Hit
CTCCAGTTCCATCCATGTTGTTGCAAATGACAAGATCTCATTCCTTTATTATGGCTGAATAGTACTCCATTGTGT106190.4355706903050964No Hit
GATTGCAGGCGTGAGCCACCGTGCCTGGCTAATTTTCGTATATTTAGTAGAGATGGGGGTTTCACCATTTTGATC100270.41128800373756486No Hit
GCTCTGAATAGCCAGAATCTCAACTACATTACCAAGTAGTGGCCCAGAGAAGTGAGAAGACAAAGAGATAGAAGA99940.4099344080336315No Hit
CTGTAAGATTCTCCCCGGGCCTGAAAGCTTAAGATGAATAACTCCTCCCTTTTCAGGCCCAGTCCCAAGGCGCAA95120.39016370714587784No Hit
GACTAACCTCGGTTGTTTTGTGTTGTGGGATTGTTCAAGTGATTCTCCAGTGTCAGCCTCCAAAGTAGCTGGGAA95000.3896714905262657No Hit
AGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACGAGGTGGCCGAATCTTCCTTCAGGATATCA90580.3715415117038857No Hit
GTGGTAGCCAGGATGGTCTCGATCTCCTGACCCCGTGATCCGTCCACCTCGGCCTCCCAAAGTGCTGGGATGACA89990.3691214466574595No Hit
GTTTATAAGTGTGTCTCACCTCCCGCTTCACCCTCTTCCTCCTTCTCTGGCTATGTAAGAAGTTCCTGCTTCCCC89210.3659220386299807No Hit
GTATGAGGCAAAAGTCATAATGGTTGTCAGGTTCAGGAGTTTGGAAGCGCATATAGTGCCTCATCTGCTGTTCCC87560.3591540601103139No Hit
GTGAAACCCCATCTCTGCTAAAAATACAAAAATTAGCTAGGGGTGGTGGGGGGCACCTGTAATCCCAGCTATTCA81730.33524053600749154No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT81530.33442017497480464No Hit
CTGTTCTTGCATTAGTTTGCTGAGGATAATGGCTTCCAGCTCCATCCATATCCCTGCCAAAAACATGATCTCATT81080.33257436265125917No Hit
AAGTAATTCTAAATTTAAGAAACACATTTGGCAAAATTATCTACAATCCTCCTGTGGGTAAAGATGCATATGTGG79400.3256833299766894No Hit
GCCTTAATATTTCTCTTTCATTCTCACCCACATGTTTTGTGCTTCTGTACAGGGAGGTCCCTTGCCGCTCTGAAA75480.3096042537360267No Hit
GGTCCCCAGGCGTGCTCGGTAGGGTCTTGACAGCACAGCCGTTTATAAGACGTCTCACCCAGACCTCACTTCCCT70920.29090002219076594No Hit
GGAGTGTAGTGGCGCGAACTCAGCTCACTGCAACCTCCGTCTCCCGGGTTCAAGCTATTCTCCTGCTTCAGCCTC69110.2834757548449497No Hit
CCCTGGGGCTCCCGGCCCCTCTGGCCCCAGCGCCGCCGGCTCCGGGGTTCACGCTCGGGGGTCCCAGCTCGAGCC66830.27412363907231935No Hit
CCCAAGTGACTCAAGGGTCTCTTCTGAGTTTTTCTCTTGCCTTCAAAGTACACCATAATGATTGGCTTTCCTGTA66490.27272902531675164No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC63170.25911103217414955No Hit
CCGTTCCCCTCTTCTGTCTCTCTCTTCCTTCACCTCCCTCTCTCCCACGTCCCCTGCATTTATCCCATGTCAGCA59700.24487776825703222No Hit
GGCTGGTCTCAAAATCGTTCAACACAGCAATACTCCTGCCTCAGCTTCCCCAAGTGCTGGGATTACAGGTGTGAG59410.24368824475963627No Hit
CGGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC58970.24188345048772514No Hit
GCCTTGTGTTTCCTCTGTTGCAGTAGTTCTGTAGCTCTGCTTTCCCAGATCATGAAGGAGACAAGTATATTAATT58040.23806877168573115No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT57960.23774062727265644RNA PCR Primer, Index 47 (96% over 28bp)
CACGTCGCTGGGCTCAGGGTGGCGGGGCGTGTTGCAGGTGTACAGTCGCACGCCGCTGGGCGGGGAGCGCGGGAA57280.23495139976152107No Hit
GTACATGGGCTTGCCGCAACACTGGCTTAAATCAGGCATCATTAACATCGTTTCCTTGTATTTTTTTTTTAGATG55560.22789629488041394No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA51340.21058667709072085No Hit
GAACATGGACCAGGGGCAGTGGTTCATGCCTGTAATCTCAGCACTTTGAGAGGCTGGGGTGGAAGGATTTCTTGA50420.20681301634036123No Hit
ACGCAGAGTACATGGGGAGGGCAGGGGCTTGTGCCCATTTCACTGTCATCCCTGCTGCATCCCAAGGACTAGAAT49050.2011935432664561No Hit
GTACAGATTATTTAATAATCACCCAAATGTTAAACCTAGTACTCATTAGTTATTACTGATCCCCTCTCTCCTCCT48750.19996300171742581No Hit
TCCAGTAAGTGACTTGGGAACAAAGGAACACATGTAAGAAGATAAGGCAGCATCAGTTCAGGAGCTTGTCTTGGA48670.1996348573043511No Hit
GTCCAGTTCCATCCATGTTGTTGCAAATGACAAGATCTCATTCCTTTATTATGGCTGAATAGTACTCCATTGTGT48030.19700970199975307No Hit
TGCTCAGTTGTGCTCTGAAGGAGTCGCTCCTGTTTGGATACTTTGGTTCTCAGTTCCAGAAGTTCCCTTTCTGTT47770.19594323265726016No Hit
TGTTTTTTTCTGGATAATTGTTTCTTGTTAATAATTAGATTAAAACATGTGGAAATTATCTCTTCCTTTTATCTC47090.19315400514612477No Hit
GGGCAGGGCAGGGCATGGTATGGCCAGTAGAGGACAGGACAAGAGCCGGTCCACAGAGAGAGCAGGACTGATGCC46860.19221058995853485No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG45390.1861809363682863No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG44340.18187404094668022No Hit
ATGGAATGGAATGGAATGGAATGGATTGGAATGGAATGGAATCAACTCGATTGCAATCGAATGGAATGGAATGGA44170.18117673406889637No Hit
CCCCACTTATAAATGAGAACATGCAGTATTTGGTTTCTGTTCCTGCATTAATTCACTTAGCATAATGGCCTCCAG43690.17920786759044788No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA43050.17658271228584987No Hit
GAGGGCAGCGGGGTCGTGTCAGACCCTATTGCACTTAGCTCCTTCCCGAATATGAGAAGATCTGCCGCATTGTCG42170.17297312374202764No Hit
CCCCCGTCTCTACAAAAAATTAAAAAAATTGGCCAGGCACAGTGGCTCACACCTGTAATCCTAACACTTTGGGAG42170.17297312374202764No Hit
GTATAAGCTAGCCTACCCAGGAGCCTGTCTCAATAACCACAGGGCAGCTGGGCATCGTCCAGTGTGGACAGACCT41890.171824618296266No Hit
ATACAAAACCATTTATTCTTCTGAATCTCTTCAAAACACTGATAACATCAACAGACAGTGTTAAATGGTAAAACT40450.1659180188609205No Hit
TCCAAGGACACCAGGGCCTTGAGGTGCAAATCTGAGTTCAGGGGGCCTTCGATGGAGGATGTGGCTTCTCAAGAT39340.16136501512950835No Hit
AAAGGATATGATTGTTTGCAGATAGGAGAAATGACTTCTCCGTGGGTACTGGGGAAGGTTTTGTGGCATCTGACT38510.1579605168438578No Hit
CTTTTTTGTTGTTGTTGTATCGTTGTCTGGTTTTGGTATCAGAATAAATCTGGGGCCAGGCATGGTAGCTCACGC37310.15303835064773658No Hit
GGAGGAAGACAACCAGAGCCATGTGAAAGAAATCATTTAAAGAGGAGCGAGTGATCCCCTATGGGGAAAAATAAA36310.14893654548430219No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGC35340.14495779447577084No Hit
ATGCTGAGCACTGTGGATGGTTTGCACCTCCTGTGACTGCTTCAGGCAGCTTCGCCAGGCTGAGCGAGCACAGGA35050.14376827097837488No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC32830.13466226351555058No Hit
TTTATAATCCTTGCTCGAGAGATCACAGGTGAAGGTTAAGGGTCTGGGTTAGCTTGCTATACAAAGTTTTGTGTG31150.1277712308409808No Hit
GCTTCTGCCTGATGGTTCGCAGCAATATATTTTGTGGTCGGTGCAGTGGTCTCGGGCTGAGCATGCTCTGTTTTG30470.12498200332984542No Hit
CTGGAGTGCAGTGGTGCCATCTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCC30210.1239155339873525No Hit
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCG30110.12350535347100905No Hit
GCAGAAAATCTCTCTATTTTTAAAAAAGCTGGCCGGGCACGGTGGCTCACACCTGTAATCCCATCACTTTGGGAG29540.12116732452785146No Hit
TGGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC29540.12116732452785146No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGC29380.12051103570170196No Hit
CAACCACTTAGTCCCTGGGGCTGCTGGCTGGGGCCATGCTTCTAAGTGCCAGTTGTTGGCTCAAGGGAGAATTTA29270.12005983713372417No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC29120.11944456635920903No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA29080.11928049415267164No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAG27760.11386611133693827No Hit
GCGCAAGCTGGAAGCTGCAGAGGACATCGCGTACCAGCTCTCACGCTCTCGGAACATCACCTACCTGCCAGCGGG26090.10701609671400286No Hit
GCCTAGACTGGTCTTGAACTCCTGGGCTCAAGTGATCTTCCTGCTTCAGCCTCCCAAAGTACTGGGATTACAGGC25680.10533435659699478No Hit
GGAAAGGAATGGAATCAACCCGATTGGAATGCAAGGGAATGGAATGGAATGAAAAGGAATGGAATGGAATCAACA25220.10344752622181495No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGG25210.10340650817018061No Hit
GTGGCATGATCATAGCTCACTGCAGCCTCAAATTCCTGGGCTGAAACAATCCTCCCACCTCAGCCTCCTGAGTAG24760.10156069584663514No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA24580.10082237091721695No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGA24530.10061728065904525No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG24420.10016608209106746RNA PCR Primer, Index 47 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)