FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810462
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC1079865.964554903665474No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT214911.1870450746825947TruSeq Adapter, Index 11 (96% over 29bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT97410.5380394617506471No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG81300.4490566496286584TruSeq Adapter, Index 11 (96% over 26bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG58910.3253865587899663No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG46530.25700622272105134No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT42960.23728749899197002No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG35590.19657965756806825No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC29580.16338371089810225No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA27230.1504035986394633No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTT25140.13885958390731204TruSeq Adapter, Index 11 (96% over 29bp)
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA23640.13057440586988295No Hit
AATCGCTTCTGACATTGCGTTTCTTGGACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACAT23550.1300772951876372No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG23240.12836502505990185No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT23200.12814408697890373TruSeq Adapter, Index 11 (95% over 23bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA23090.12753650725615892TruSeq Adapter, Index 11 (96% over 30bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG22890.12643181685116836TruSeq Adapter, Index 11 (96% over 26bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG19060.10527699559559935No Hit
TGAGAGAGACAGAAGGGGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT18880.10428277423110785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)