FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506600
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC1114387.396654719235364No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT544523.61423071817337RNA PCR Primer, Index 26 (100% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG97940.6500730120801805RNA PCR Primer, Index 26 (100% over 25bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG68390.4539360148679146No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA59800.3969202177087482No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT56080.37222885968405683RNA PCR Primer, Index 26 (100% over 28bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG54470.36154254613035974No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCC52320.34727200318598167No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATTCC39870.26463560334528075No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT37660.24996681269082704No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTTTGCC35770.23742200982344353No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTG35060.23270941192088146No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA33210.22043010752688172RNA PCR Primer, Index 26 (100% over 29bp)
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC29760.19753086419753088No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGGC29670.19693349263241738No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG29520.19593787335722818RNA PCR Primer, Index 26 (100% over 25bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTG25610.1699853975839639No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTGGTATGCC24360.16168857029072084No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATTCCGTCTTCTGCTT24190.16056020177883978RNA PCR Primer, Index 26 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG23250.15432098765432098RNA PCR Primer, Index 26 (100% over 29bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTATGCTT21100.14005044470994293RNA PCR Primer, Index 26 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA21070.13985132085490506RNA PCR Primer, Index 26 (100% over 29bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTGTGCTT16450.10918624717907872RNA PCR Primer, Index 26 (96% over 28bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTT16300.10819062790388956RNA PCR Primer, Index 26 (96% over 28bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGGATGCC16020.10633213859020312No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA15710.10427452542147882RNA PCR Primer, Index 26 (100% over 29bp)
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG15180.10075667064914377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)