FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049854
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT448804.274880126188975RNA PCR Primer, Index 27 (96% over 29bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC445924.247447740352468No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG110651.0539560738921794RNA PCR Primer, Index 27 (96% over 26bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT53970.5140714804153721RNA PCR Primer, Index 27 (96% over 29bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG53870.5131189670182711No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41410.3944357977394952RNA PCR Primer, Index 27 (96% over 30bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT34430.3279503626218503No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA31880.30366127099577656RNA PCR Primer, Index 27 (96% over 30bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG30300.2886115593215819RNA PCR Primer, Index 27 (96% over 26bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA28840.2747048637239083No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC27790.2647034730543485No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA23690.22565042377321037RNA PCR Primer, Index 27 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT22410.21345825229031845No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG19090.18183480750656758RNA PCR Primer, Index 27 (96% over 30bp)
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG18030.1717381654972977No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA17530.16697559851179308RNA PCR Primer, Index 27 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG17380.16554682841614168RNA PCR Primer, Index 27 (96% over 30bp)
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACT16640.1584982292775948No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA12680.12077869875239794No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACT12600.1200166880347172No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA12400.11811166124051535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)