FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471223
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC346292.353756024749477No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT312692.125374603306229TruSeq Adapter, Index 3 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG57940.3938220106673156TruSeq Adapter, Index 3 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG57330.3896758003375423TruSeq Adapter, Index 3 (95% over 24bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG44670.30362494332946127No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA44340.30138191151171506No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT31500.21410758260304522TruSeq Adapter, Index 3 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA30800.20934963632297754No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA29800.20255257020859516No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG28010.1903858218638507No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG26900.18284107847688624RNA PCR Primer, Index 3 (96% over 28bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA22260.15130269170615196No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA21930.14905965988840578TruSeq Adapter, Index 3 (96% over 28bp)
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG18290.12431833923205388No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA17910.12173545410858856TruSeq Adapter, Index 3 (96% over 28bp)
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA16030.10895696981354967No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT15340.10426699419462584No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG14870.10107237312086612TruSeq Adapter, Index 3 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)