FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806854
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC836784.631143412804798No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTT798304.418176565455759TruSeq Adapter, Index 8 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTG137580.7614339620135329TruSeq Adapter, Index 8 (96% over 25bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG94130.52096074170907No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTT63770.35293388397734404TruSeq Adapter, Index 8 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG56210.31109320398881146TruSeq Adapter, Index 8 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA55190.3054480328792476No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA53840.29797648288129536TruSeq Adapter, Index 8 (96% over 29bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT53580.2965375176965045No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA52720.2917778636237349No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATG52670.2915011395497367No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTG38450.21280081290463979TruSeq Adapter, Index 8 (96% over 25bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA36580.20245133253710595TruSeq Adapter, Index 8 (96% over 29bp)
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCA35990.1991859884639268No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG28140.15574030884620452No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG25880.14323238070148445RNA PCR Primer, Index 8 (96% over 29bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCA25360.14035445033190286No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA24600.13614824440712972TruSeq Adapter, Index 8 (96% over 29bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTG23800.13172065922315804TruSeq Adapter, Index 8 (96% over 29bp)
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA23180.12828928070557996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)