FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855695
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC2528308.853536529636393No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT900743.154188384964081RNA PCR Primer, Index 26 (100% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG218060.7635969527558091RNA PCR Primer, Index 26 (100% over 25bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG142340.49844258578034417No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCC114050.39937738448959004No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT101600.3557802916628001RNA PCR Primer, Index 26 (100% over 28bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATTCC93360.32692566958306124No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTTTGCC79920.2798618199772735No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGGC67050.23479398185030265No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT65090.22793050378279192No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG60680.21248767813089287No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTGGTATGCC55990.19606435561220648No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG55450.1941733973691168RNA PCR Primer, Index 26 (100% over 25bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA51840.18153199133660983RNA PCR Primer, Index 26 (100% over 29bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATTCCGTCTTCTGCTT42900.1502261270899028RNA PCR Primer, Index 26 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA40410.1415067085245448RNA PCR Primer, Index 26 (100% over 29bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT39270.13751468556691102No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGGATGCC35890.1256786876749793No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTATGCTT33830.11846503215504457RNA PCR Primer, Index 26 (96% over 28bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA32270.11300226389722992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)