FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095965
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC63480.579215577139781No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT49360.45037934605575913RNA PCR Primer, Index 17 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG16270.14845364587372772RNA PCR Primer, Index 17 (96% over 28bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAACCAAAGCTAAGACCCCCGAAACC15400.1405154361681258No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG13500.12317911612140899RNA PCR Primer, Index 17 (95% over 24bp)
GTATCAACGCAGAGTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCT11080.10109811900927493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)