FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539723
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC317982.065176658398946No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTT246181.5988590155501998TruSeq Adapter, Index 1 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG53870.3498681256303893TruSeq Adapter, Index 1 (96% over 25bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA32650.2120511286770413No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG30560.19847725857183401TruSeq Adapter, Index 1 (96% over 29bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG29430.19113827617045404No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG29350.1906187021951351No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG24430.15866490271302047No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTT22090.14346736393494156TruSeq Adapter, Index 1 (96% over 28bp)
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC20790.1350242868360088No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAA18150.11787834565048388TruSeq Adapter, Index 1 (96% over 29bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15890.1032003808477239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)