FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113420
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC278662.502739307718561No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT211561.900091609635178RNA PCR Primer, Index 17 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG46460.41727290689946295RNA PCR Primer, Index 17 (95% over 24bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG28040.2518366833719531No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT22150.19893660972499144RNA PCR Primer, Index 17 (96% over 27bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17600.15807152736613317No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAACCAAAGCTAAGACCCCCGAAACC17520.15735302042355984No Hit
GTATCAACGCAGAGTACATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC13740.12340356738696988No Hit
GTATCAACGCAGAGTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCT13520.1214276732948932No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA13160.11819439205331321No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG13010.11684719153598822RNA PCR Primer, Index 17 (95% over 24bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA11970.1075066012825349RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAA11370.1021177992132349No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG11240.10095022543155324RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)