FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307389
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC327812.507363913877201No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT325142.4869415300266406RNA PCR Primer, Index 33 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG73150.5595121268421258RNA PCR Primer, Index 33 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41560.31788549544167805RNA PCR Primer, Index 33 (96% over 29bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG39650.30327622459726983No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT34960.26740319828299RNA PCR Primer, Index 33 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCA27160.20774230164090413No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA26290.20108781701544068No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC26190.20032293372515755No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT23220.17760590000374793No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA22200.1698040904428598RNA PCR Primer, Index 33 (96% over 29bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG19070.1458632434569971RNA PCR Primer, Index 33 (96% over 25bp)
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAG17930.1371435739477692No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG17870.1366846439735993RNA PCR Primer, Index 33 (96% over 29bp)
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG16880.12911229939979607No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA16320.12482895297421043RNA PCR Primer, Index 33 (96% over 29bp)
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA14320.10953128716854738No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA14180.10846045056215098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)