FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486066
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC933976.284848721389224No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT358422.4118713435338672TruSeq Adapter, Index 3 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG72160.4855773565911608TruSeq Adapter, Index 3 (95% over 24bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG67320.4530081436490707No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA67310.45294085188679367No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA46290.31149356758044394No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG42430.28551894734150435No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG38490.2590059930043484TruSeq Adapter, Index 3 (96% over 28bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA36790.24756639341725067No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT33550.22576386243948787TruSeq Adapter, Index 3 (96% over 27bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA27080.1822260922462394TruSeq Adapter, Index 3 (96% over 28bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT25050.16856586450399916No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA21410.14407166303515456No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA20590.13855373852843683TruSeq Adapter, Index 3 (96% over 28bp)
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGT19130.12872914123598816No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG19070.1283253906623259No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG18440.12408600963887202TruSeq Adapter, Index 3 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG16330.10988744779841542RNA PCR Primer, Index 3 (96% over 28bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCAC15660.10537889972585335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)