FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970205
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC196912.029571070031591No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT173981.79322926597987TruSeq Adapter, Index 11 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG49410.5092738132662685TruSeq Adapter, Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG23390.24108307007281965TruSeq Adapter, Index 11 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA21330.21985044397833448No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG20010.20624507191779057No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT17910.1846001618214707TruSeq Adapter, Index 11 (96% over 27bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA15970.16460438773248953No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA14470.14914373766368963TruSeq Adapter, Index 11 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAG13210.13615679160589772No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT12380.12760189856782844No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTAC11900.1226544905458125No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG11780.12141763854030849TruSeq Adapter, Index 11 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG9930.10234950345545529RNA PCR Primer, Index 11 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAA9820.10121572245040997TruSeq Adapter, Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)