FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260696
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC260982.0701263429089964No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT161361.2799279128354495RNA PCR Primer, Index 46 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG33470.26548826997150776RNA PCR Primer, Index 46 (95% over 24bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA26150.20742510486271076No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG22970.18220094297118417RNA PCR Primer, Index 46 (96% over 28bp)
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG22960.18212162170737434No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG19440.15420053684631346No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG15610.12382049280714781No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGA14680.11644361527283341No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT14410.11430194114996794RNA PCR Primer, Index 46 (96% over 27bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAACCAAAGCTAAGACCCCCGAAACC13050.1035142492718308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)