FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004048266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492806
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC1255873.5955904794025204No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT701872.0094731857423516TruSeq Adapter, Index 3 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG167370.47918493039693594TruSeq Adapter, Index 3 (95% over 24bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG87220.24971326778527062No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG83530.2391486959195558No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCCAGTCAAAGCTTCGTATACGGGGATGGAGACACCTACTTGA81660.23379483429655126No Hit
GTCCAAGGGTGACGGCCAGGGAGAGTGGTGACTGAGTCATCACAACATCCCCACTGCATCCTGGGACCCAGAGCA76570.21922202378259772No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT72560.20774128308299974TruSeq Adapter, Index 3 (96% over 27bp)
GTAATAGACAGCCGTGTCTTCGTCTCTCAGACTGTTCATTTGCAGATACACTGACATCTTGGCGTTGTCTCTGGA71060.20344674167417257No Hit
GTCTGGGACCCCGGAGTCTCGCTTAGAAACCTTATAAATTAGGCGCCTGGGAGATTGGCCTGGCCTCTGCTGAAA70110.200726865448582No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66820.19130750462522111No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAG62110.17782264460150377No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT62020.17756497211697417No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCTCTGATTTCACACTGAAAATCAGCAGCGTGGAGGCTGAGGAT60530.1732990609842058No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA58150.16648505528220003No Hit
GTCTATTACTGTGCGAGAGATCTCGATCACTATAATTCGGAGAACTATCCCCAAAGTTACTACGATCTGGACGTC57820.16554025617225807No Hit
TTATTACTGCATGCAAGCTACAAACTGGCCTCCGATTTACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACG57650.16505354147925763No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG57290.16402285154113913No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACGCTGCTGATTTTCAGTGTGAAATCAGAGCCTGACCCACTGCCG55380.1585544688138992No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT52010.14890606578206747No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGG51840.14841935108906706No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC49550.14186301787159092No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA48930.14008794075594236TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCT47330.1355070965865267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC46780.1339324314032901No Hit
CTCCATCCCCGTATACGAAGCTTTGACTGGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGCCAGGG45030.1289221330929917No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTCTCCATCCCCGTATACGAAGCTTTGACTGGACCTGCAGG44430.12720431652946085No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43120.12345375036575178No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTC41260.11812851901880608No Hit
GGCCAGGCCAATCTCCCAGGCGCCTAATTTATAAGGTTTCTAAGCGAGACTCCGGGGTCCCAGACAGATTCAGCG41130.11775632543004105No Hit
GGGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCCAGGCGCCTAATTTATAAGGTTT40740.11663974466374599No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCA40660.11641070245527521No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAATCGGAGG40460.11583809693409826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTC40400.11566631527774518No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCACCACTC39780.1138912381620966No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG39330.11260287573944847TruSeq Adapter, Index 3 (95% over 24bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA38130.10916724261238672TruSeq Adapter, Index 3 (96% over 28bp)
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC37390.10704860218403198No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC37370.10699134163191429No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATA37360.10696271135585543No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG37200.10650462693891387No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT36910.10567434893320729No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTATTATGTGACCTGGATCC36550.10464365899508876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAG36520.10455776816691222No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCACCACTCTCCCTGGCCGTCACCCT36470.10441461678661798No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT36430.1043000956823826No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAATCGGAGGCCAGTTTGTAGCTT36170.10355570850485254No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35720.10226734608220439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)