FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686339
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC387742.2993004372193253No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT346812.056585301057498RNA PCR Primer, Index 39 (96% over 29bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG85870.5092095954609365RNA PCR Primer, Index 39 (96% over 26bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG46290.274499967088468No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT37480.2222566162556876RNA PCR Primer, Index 39 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG32710.19397048873328554RNA PCR Primer, Index 39 (96% over 30bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT26240.1556033514020609No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA25350.15032564626685382RNA PCR Primer, Index 39 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA24220.1436247397468718No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA23820.14125273743891353No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG22520.13354372993804922RNA PCR Primer, Index 39 (96% over 26bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAA19870.11782921464782586RNA PCR Primer, Index 39 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC17390.10312280033848473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)