FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622463
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCC950925.860965704610829No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT896825.5275220451868545TruSeq Adapter, Index 4 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG270811.6691289724326535TruSeq Adapter, Index 4 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT170061.0481594957789484TruSeq Adapter, Index 4 (96% over 27bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG90580.5582869994569984TruSeq Adapter, Index 4 (95% over 24bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGT86380.5324004307031963No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG81930.5049729947616679No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTG55300.34083982192506085TruSeq Adapter, Index 4 (96% over 28bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA49000.3020099687943577TruSeq Adapter, Index 4 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA44400.2736580125401935TruSeq Adapter, Index 4 (96% over 28bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA36450.2246584359704967TruSeq Adapter, Index 4 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG34240.2110371700309961No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA27820.17146770065018432TruSeq Adapter, Index 4 (96% over 28bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATATCGTATGCCGTCTTCTGCTT22440.13830823877031403RNA PCR Primer, Index 36 (96% over 27bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA20330.12530331970590391TruSeq Adapter, Index 4 (96% over 28bp)
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGC19270.11877004282994436TruSeq Adapter, Index 4 (96% over 25bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT17910.1103877253287132No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTG16420.10120415688986437TruSeq Adapter, Index 4 (96% over 28bp)
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC16310.10052617532726477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)