FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574513
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCC756454.804342676116361RNA PCR Primer, Index 32 (95% over 21bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT447302.840878417644059RNA PCR Primer, Index 32 (96% over 32bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG160351.018410136975687RNA PCR Primer, Index 32 (96% over 29bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT63930.4060303090542917RNA PCR Primer, Index 32 (96% over 32bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG45790.290820082146035RNA PCR Primer, Index 32 (95% over 22bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG40300.25595215790533327RNA PCR Primer, Index 32 (96% over 29bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT38950.2473780781740132RNA PCR Primer, Index 32 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG32280.20501577313112054No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA32150.2041901210088453RNA PCR Primer, Index 32 (96% over 33bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG28940.1838028647588175RNA PCR Primer, Index 32 (96% over 33bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA24410.15503206388261004RNA PCR Primer, Index 32 (96% over 33bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTCTTCTGCTT17050.10828745142148716TruSeq Adapter, Index 4 (96% over 25bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCC17000.10796989291291974No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA16410.10422270251182429RNA PCR Primer, Index 32 (96% over 33bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT15780.10022146530387491No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC15780.10022146530387491No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)