FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253240
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCC676465.397689189620504No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT483923.861351377230219Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG158851.267514602151224Illumina Paired End PCR Primer 2 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT72400.5777025948740864Illumina Paired End PCR Primer 2 (96% over 27bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG50330.4015990552487951No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG46760.3731128913855287Illumina Paired End PCR Primer 2 (95% over 24bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGT44590.35579777217452363No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA30410.2426510484823338Illumina Paired End PCR Primer 2 (96% over 28bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTG30210.24105518496058218Illumina Paired End PCR Primer 2 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA23490.18743417062972775Illumina Paired End PCR Primer 2 (96% over 28bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA17590.14035619673805497Illumina Paired End PCR Primer 2 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATGTCGTATGCCGTCTTCTG16760.13373336312278572TruSeq Adapter, Index 1 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG14570.11625865755960549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)