FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946545
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCC631706.673745041176067No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTT276402.9200936035793332TruSeq Adapter, Index 1 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTG109641.1583178824039004TruSeq Adapter, Index 1 (95% over 24bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG49120.5189399341816818No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGT34790.3675472375851122No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA20190.2133020617086351TruSeq Adapter, Index 1 (96% over 28bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTG20140.21277382480494852TruSeq Adapter, Index 1 (96% over 28bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTT16810.17759324701942328TruSeq Adapter, Index 1 (96% over 27bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA16410.17336735178993073TruSeq Adapter, Index 1 (96% over 28bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTG15730.16618332989979345TruSeq Adapter, Index 1 (95% over 24bp)
TGGCCTCCCGGGAAGGAGCGCCCTGGCGGAGAAGACTCGAACGGCTCCCACAGCCGGGCGTTGGGGGAAAGCTAC15380.1624856715739875No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA10910.11526129238440856TruSeq Adapter, Index 1 (96% over 28bp)
CACCAAATCTCAGGTTTAGCCTCTAAAGCGCAAAGGTTTTCAGGGTGAGGTTTGACTCAGTAGGCCTTTCAAAGT10660.11262010786597573No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG9820.10374572788404143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)