FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487257
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTT682872.7454742312515354RNA PCR Primer, Index 45 (96% over 29bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCC595802.3954098832569373No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTG195680.786730120771597RNA PCR Primer, Index 45 (96% over 26bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTT147840.5943897232975925RNA PCR Primer, Index 45 (96% over 29bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTG70750.2844498980201885RNA PCR Primer, Index 45 (96% over 26bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGT67550.2715843195938337No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG54210.21795093952896705No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTG46380.18647047731698013RNA PCR Primer, Index 45 (96% over 30bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA38820.15607554828471684RNA PCR Primer, Index 45 (96% over 30bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA35110.14115951829666173RNA PCR Primer, Index 45 (96% over 30bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG34470.13858640261139077No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA26990.10851311303978639RNA PCR Primer, Index 45 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)