FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478916
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCC756803.0529473366584425No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTT691602.7899291464494964TruSeq Adapter, Index 6 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTG245780.9914817605759937TruSeq Adapter, Index 6 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTT111420.4494706557220979TruSeq Adapter, Index 6 (96% over 27bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCG76240.3075537856062892No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTG71000.28641551387784014TruSeq Adapter, Index 6 (95% over 24bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGT67060.27052146986828113No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTG47510.19165635301881953TruSeq Adapter, Index 6 (96% over 28bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAA45810.18479851677104026TruSeq Adapter, Index 6 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG38730.15623764580970068No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAA38380.15482573834692262TruSeq Adapter, Index 6 (96% over 28bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAA27050.10912027676613487TruSeq Adapter, Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)