FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282712
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCC936134.100955354858607RNA PCR Primer, Index 32 (95% over 21bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT773853.39004657617781RNA PCR Primer, Index 32 (96% over 32bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG254021.1127991616988915RNA PCR Primer, Index 32 (96% over 29bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT107120.4692663813919583RNA PCR Primer, Index 32 (96% over 32bp)
GAGGGACTCGCTGGCAGCAGCCCCAGCCTGACCGCTCGGAGTGTACTTTCCTTGACAGGCAAGGCTGTTGGCCAG73560.32224827310672566No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG69920.3063023281079698RNA PCR Primer, Index 32 (96% over 29bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG69420.30411195104770117RNA PCR Primer, Index 32 (95% over 22bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT66900.2930724506639471RNA PCR Primer, Index 32 (95% over 23bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA53240.23323134937740722RNA PCR Primer, Index 32 (96% over 33bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG48110.21075808073905072RNA PCR Primer, Index 32 (96% over 33bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA40860.17899761336515513RNA PCR Primer, Index 32 (96% over 33bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG30130.1319921216517896No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA29330.12848751835535976RNA PCR Primer, Index 32 (96% over 33bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTCTTCTGCTT27070.1185870140429454TruSeq Adapter, Index 4 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)