FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669231
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCC551623.3046354878384117No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT412492.4711379072159576Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG128410.7692763913442777Illumina Paired End PCR Primer 2 (95% over 24bp)
GTACAGGGGGCTGGCCTGGCCTCTGCTGATACCAGGAAACATATTTATTCGCCATTCTATCTCCAGAGCAGGTGA58940.35309672537833287No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT58890.3527971862492369Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG53690.3216451168232557No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC51960.31128106295653507No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT49290.2952856734628101No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAGTGCTGCTGTCCCACGCCTGACAG48890.2928893604300423No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG46790.28030871700801147No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGATAAGGGATC45500.27258060747733537No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGTGGTCTTCGGCGGAGGGACCAAGCT44920.2691059535798221No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA43130.2583824527581863No Hit
CCTATGAGCTGACTCAGACACCCTCAGCGTCCGTGTCCCCAGGGCAGACAGCCAGCATCACCTGCTCTGGAGATA42780.2562856788545145No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42400.2540091814733851No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGC41500.2486174771496575No Hit
CCCCTGTACTGGTCATCTATGAAGATAGTACGCGGCCCTCAGGGATCCCTTATCGATTCTCTGGCTCCAACTCTG41020.2457419015103362No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40750.24412439021321794No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40470.2424469710902805No Hit
GGCCAGGCCAGCCCCCTGTACTGGTCATCTATGAAGATAGTACGCGGCCCTCAGGGATCCCTTATCGATTCTCTG38190.22878798680350415No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG37230.22303683552486142Illumina Paired End PCR Primer 2 (95% over 24bp)
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG36140.21650688251056927No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGT34660.20764052428932844No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG34150.20458522517254948No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG33190.19883407389390684No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC32700.19589859042876628No Hit
GGCCTGGCCTCTGCTGATACCAGGAAACATATTTATTCGCCATTCTATCTCCAGAGCAGGTGATGCTGGCTGTCT32660.19565895912548953No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTC31860.19086633305995396No Hit
GCTCAGGCGTCAGGCTCAGATAACTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30390.18205988266453235No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG29850.17882486007029583No Hit
CCCTCAGCGTCCGTGTCCCCAGGGCAGACAGCCAGCATCACCTGCTCTGGAGATAGAATGGCGAATAAATATGTT29390.1760691000826129No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCTGG29270.17535020617278257No Hit
GTACTGGTCATCTATGAAGATAGTACGCGGCCCTCAGGGATCCCTTATCGATTCTCTGGCTCCAACTCTGGGAAC29150.17463131226295223No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG28580.17121656619125813No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG28400.17013822532651263No Hit
GGCCAGCCCCCTGTACTGGTCATCTATGAAGATAGTACGCGGCCCTCAGGGATCCCTTATCGATTCTCTGGCTCC28090.16828108272611758No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27970.16756218881628726No Hit
GACCAGTACAGGGGGCTGGCCTGGCCTCTGCTGATACCAGGAAACATATTTATTCGCCATTCTATCTCCAGAGCA27930.16732255751301048No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27870.16696311055809532No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGA27270.16336864100894363No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAA27160.1627096549249325No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGTTATCTGAGCCTGACGCCTGAGCAGTGGA27040.16199076101510215No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC26950.16145159058272943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCT26800.16055297319544148No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG26690.15989398711143035No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGATAAGGGATCCCTGAGGGCCGCGTACTATCTTCATAGA26580.1592350010274192No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCA26180.15683868799465142No Hit
GCCCTCAGGGATCCCTTATCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCA26180.15683868799465142No Hit
GTATCAACGCAGAGTACATGGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCT26060.1561197940848211No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGC25560.15312440279386136No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTG25490.152705048013127Illumina Paired End PCR Primer 2 (96% over 28bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA25000.14976956454798648Illumina Paired End PCR Primer 2 (96% over 28bp)
CTCCAGAGCAGGTGATGCTGGCTGTCTGCCCTGGGGACACGGACGCTGAGGGTGTCTGAGTCAGCTCATAGGAGG24990.14970965672216727No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC24900.14917048628979454No Hit
GATAACTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGC24160.14473730717917413No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23580.14126265328166082No Hit
GTATCAACGCAGAGTACATGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTC23580.14126265328166082No Hit
CCCCAGGGCAGACAGCCAGCATCACCTGCTCTGGAGATAGAATGGCGAATAAATATGTTTCCTGGTATCAGCAGA23290.13952532633290418No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAGCTGACT23110.1384469854681587No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22960.13754836808087076No Hit
GCTCATAGGAGGCCACGGATCCTGTGCAGTAAGCAAGGACGCCGAGGAAGAGAGGGATCCATGCCATGGCTGAGG22910.1372488289517748No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT22670.13581104113211412No Hit
GCTCTGGAGATAGAATGGCGAATAAATATGTTTCCTGGTATCAGCAGAGGCCAGGCCAGCCCCCTGTACTGGTCA22370.1340138063575383No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGG22220.13311518897025038No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAA22160.13275574201533522No Hit
GGGCTGGCCTGGCCTCTGCTGATACCAGGAAACATATTTATTCGCCATTCTATCTCCAGAGCAGGTGATGCTGGC22090.13233638723460084No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAG22070.13221657158296243No Hit
TCTATGAAGATAGTACGCGGCCCTCAGGGATCCCTTATCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTC21930.13137786202149374No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT21570.12922118029200272No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG21420.1283225629047148No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC21080.1262856968268622No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAG20910.12526726378793587No Hit
GTATCAACGCAGAGTACATGGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCT20720.12412901509737118No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG20520.12293085858098729No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCA20450.12251150380025293No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC20400.12221196467115696No Hit
GACTCAGACACCCTCAGCGTCCGTGTCCCCAGGGCAGACAGCCAGCATCACCTGCTCTGGAGATAGAATGGCGAA20070.12023500641912352No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA19770.1184377716445477Illumina Paired End PCR Primer 2 (96% over 28bp)
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG19710.11807832468963253No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGACACCCTCAGCGTCCGTGTCCCCAGGGCAGACAGCCAGCA19670.11783869338635575No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCC19640.11765896990889815No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC19330.11580182730850314No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAC19150.11472348644375764No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG19000.11382486905646971No Hit
GTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCT18970.11364514557901213No Hit
GGAGATAGAATGGCGAATAAATATGTTTCCTGGTATCAGCAGAGGCCAGGCCAGCCCCCTGTACTGGTCATCTAT18800.11262671254008583No Hit
CTTCATAGATGACCAGTACAGGGGGCTGGCCTGGCCTCTGCTGATACCAGGAAACATATTTATTCGCCATTCTAT18790.11256680471426664No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTA18580.11130874037206355No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGACACCCTCAGCGTCCGTGTCCCCAGGGCAGACAGCCAGCATCACC18540.11106910906878677No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCTGGCCGCGTACTTGTTGTTGCT18490.1107695699396908No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAG18300.1096313212491261No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGC18280.10951150559748771No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCC18260.10939168994584933No Hit
GGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGAC18240.10927187429421092No Hit
GCAGTTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGA18120.1085529803843806No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAG18090.10837325690692301No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCT18030.10801380995200784No Hit
GGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGT17820.10675574560980476No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC17760.10639629865488959No Hit
CAGCAGCACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCAC17500.10483869518359054No Hit
GCCCTGGGGACACGGACGCTGAGGGTGTCTGAGTCAGCTCATAGGAGGCCACGGATCCTGTGCAGTAAGCAAGGA17390.1041797090995794No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGA17140.10268201345409952No Hit
GCAGAGGCCAGGCCAGCCCCCTGTACTGGTCATCTATGAAGATAGTACGCGGCCCTCAGGGATCCCTTATCGATT17060.10220275084754596No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG16970.1016635804151732No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAACTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG16870.10106450215698126No Hit
GCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGATAA16860.10100459433116206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)