FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706990
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCC756284.430488755060077No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT721574.227148372281032TruSeq Adapter, Index 4 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG225111.3187540641714364TruSeq Adapter, Index 4 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTT128330.7517911645645259TruSeq Adapter, Index 4 (96% over 27bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG76790.4498561795909759No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTG74430.43603067387623834TruSeq Adapter, Index 4 (95% over 24bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGT66560.38992612727666826No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTG44830.26262602592868145TruSeq Adapter, Index 4 (96% over 28bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA44250.2592282321513307TruSeq Adapter, Index 4 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG43020.25202256603729367No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA36740.2152326609997715TruSeq Adapter, Index 4 (96% over 28bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA27410.16057504730549094TruSeq Adapter, Index 4 (96% over 28bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT24170.14159426827339353No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC20680.12114892295795522No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATATCGTATGCCGTCTTCTGCTT19310.11312309972524737RNA PCR Primer, Index 36 (96% over 27bp)
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAA19050.11159995079057289TruSeq Adapter, Index 4 (96% over 28bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18560.10872940087522483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)