FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452944
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC617884.252607120439604No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTT552283.80111002213437TruSeq Adapter, Index 7 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTG177841.2239976213811405TruSeq Adapter, Index 7 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTT99520.6849541345020868TruSeq Adapter, Index 7 (96% over 27bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTG59880.41212875375788743TruSeq Adapter, Index 7 (95% over 24bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT53860.3706956358951205No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG44550.30661883733991124No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG44070.3033152000352388No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA32210.22168782829895717TruSeq Adapter, Index 7 (96% over 28bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTG31300.21542468257551564TruSeq Adapter, Index 7 (96% over 28bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT23310.16043288660815558No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC21180.1457729960686716No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA20930.14405235163915472TruSeq Adapter, Index 7 (96% over 28bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18750.12904833221376735No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA15510.10674878040722836TruSeq Adapter, Index 7 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATGTCGTATGCCGTCTTCTG14650.10082976356969023RNA PCR Primer, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)