FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107748
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCC409933.7005708879636883No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT288912.6080841491025035Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG99080.8944272524075872Illumina Paired End PCR Primer 2 (95% over 24bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTT42360.38239744057312675Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTG29110.2627853988452247Illumina Paired End PCR Primer 2 (95% over 24bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG27260.24608484962283841No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG27080.24445993132011973No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGT24600.22207216803821808No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA19770.17847019358193378Illumina Paired End PCR Primer 2 (96% over 28bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTG19390.1750398104984166Illumina Paired End PCR Primer 2 (96% over 28bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA15400.1390207881214861Illumina Paired End PCR Primer 2 (96% over 28bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT13490.12177859946486023No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC13190.11907040229366245No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAA11400.10291149250551568Illumina Paired End PCR Primer 2 (96% over 28bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATGTCGTATGCCGTCTTCTG11080.10002274885623806TruSeq Adapter, Index 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)