FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565548
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG8870.15683903046248948No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTT8400.14852850686413885Illumina PCR Primer Index 6 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG6550.11581687142382256Illumina PCR Primer Index 6 (96% over 27bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT6250.11051228189296045No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGC5680.10043356178432246No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)