FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800372
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC86231.0773740210801976No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTT70040.8750930817170016TruSeq Adapter, Index 7 (96% over 27bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTG24860.31060556841068904TruSeq Adapter, Index 7 (95% over 24bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG20580.2571304343480281No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTT12150.15180441094890876TruSeq Adapter, Index 7 (96% over 27bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT10490.13106405521432532No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC10070.1258164953296717No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)