FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280020
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTT539572.366514328821677Illumina PCR Primer Index 7 (96% over 26bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCC492672.1608143788212386No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTG183340.8041157533705845Illumina PCR Primer Index 7 (95% over 23bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTT93260.40903149972368663Illumina PCR Primer Index 7 (96% over 26bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTG56360.24719081411566562Illumina PCR Primer Index 7 (95% over 23bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG48790.2139893509706055No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGT48780.2139454917062131No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTG39400.17280550170612538Illumina PCR Primer Index 7 (96% over 27bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA34100.14956009157814407Illumina PCR Primer Index 7 (96% over 27bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA28360.12438487381689634Illumina PCR Primer Index 7 (96% over 27bp)
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGC25030.10977973877422129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)