FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654399
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCC539313.259854484921715RNA PCR Primer, Index 32 (95% over 21bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT467552.826101804945482RNA PCR Primer, Index 32 (96% over 32bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG153330.9268018174575782RNA PCR Primer, Index 32 (96% over 29bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTT63520.3839460734683713RNA PCR Primer, Index 32 (96% over 32bp)
GTGTGATCATGGCTCACTGCCTCAACCTCTCCAGGCTCAGGTGATTCTCCCACCTCAGCCTCCTGAGTAGCTGGG60780.367384167906291No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTG43560.26329803149058967RNA PCR Primer, Index 32 (96% over 29bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG43310.26178690872032684RNA PCR Primer, Index 32 (95% over 22bp)
ATACCACTCTCTCCAATGTCCATGGCTCTCTGGAGATTTTGCCAGTTCTATCACTGCATTGTTTGAAGAAATGCT38360.2318666778691235No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT38230.2310808940285868RNA PCR Primer, Index 32 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG31780.19209392655580665No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA31540.1906432486963544RNA PCR Primer, Index 32 (96% over 33bp)
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG30330.18332941448828247RNA PCR Primer, Index 32 (96% over 33bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA24990.1510518321154691RNA PCR Primer, Index 32 (96% over 33bp)
GGGAGGTAGACGAGCTGTTCGACGTAAAGAACGCCTTCTACATCGGGAGCTACCAGCAGTGCATAAACGAGGCGC24170.14609534942900715No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT18330.11079552151566822No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA17430.10535547954272216RNA PCR Primer, Index 32 (96% over 33bp)
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTCTTCTGCTT16900.10215189926976503TruSeq Adapter, Index 4 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)