FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947809
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCC1241706.374855029420236No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTT741183.8051985589962873Illumina PCR Primer Index 10 (96% over 26bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTG287201.4744772203024012Illumina PCR Primer Index 10 (95% over 23bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTT113830.5844002158322505Illumina PCR Primer Index 10 (96% over 26bp)
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCG82650.424322918725604No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTG77610.3984476917397959Illumina PCR Primer Index 10 (95% over 23bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGT74860.38432926431698383No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTG53340.27384615226646963Illumina PCR Primer Index 10 (96% over 27bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA48520.24910039947448645Illumina PCR Primer Index 10 (96% over 27bp)
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA39020.2003276501956814Illumina PCR Primer Index 10 (96% over 27bp)
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA27820.1428271457827744Illumina PCR Primer Index 10 (96% over 27bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA20250.1039629655679792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)